In vitro fertilisation


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In vitro fertilisation (IVF) is used to re-animate strains from the frozen state from sources such as the repository of the Australian Phenome Bank. The team is using the latest and most efficient IVF techniques and re-animation success is currently running at 97% of attempts with sperm thawed from the APB archive.

This IVF process:

  • Incubation of the frozen-thawed sperm with oocytes harvested from females of the correct background. Resulting two-cell embryos are transferred into pseudo-pregnant females and if successful, pups will be born 20-21 days later.

Until recently, IVF with sperm and oocytes on the C57BL/6 background was notoriously difficult. A new IVF technique developed by Professor Naomi Nakagata and colleagues in 2012 allowed the APB to change their IVF procedure to one resulting in higher two-cell fertilisation efficiency for strains on C57BL/6 background. While strain-to-strain variation is still evident, the APB consistently achieves two-cell fertilisation efficiencies of more than 80 per cent.

How to order

The Australian Phenome Bank website handles all ordering requests and can provide further information. Please visit the APB website via this link.

Updated:  21 September 2018/Responsible Officer:  Director/Page Contact:  Site manager