Congenic strains are produced by transferring the transgene/KO allele to a new genetic background strain that is more appropriate for phenotypic analysis. Utilising the traditional, random backcrossing methods it can take over 3 years to produce a congenic strain containing 99% of the recipient genome.
The APF speed congenic service:
- Utilises marker-assisted accelerated backcrossing as a congenic breeding strategy.
- Uses a genome scanning set of approximately 100 single nucleotide (SNP)-based markers
- Evaluates strain-specific polymorphisms at each backcross generation
- Preselects the founders of each generation to bias for a new genetic background
- Establishes a congenic strain within 18 months.
How to order
To initiate a project please provide details to the contact.