The APF has recently acquired new high throughput automated equipment to streamline the processing of enriched DNA libraries for their exome sequencing pipeline. This equipment was funded by an ANU Major Equipment Grant awarded to Prof. Chris Goodnow and in-kind contributions from several research groups within the ANU.
The new high throughput systems will upgrade existing facilities for DNA sequence analysis, by removing the reliance on manual processing of each DNA sample for massively parallel sequencing and comprises:
- Qiagen QIASymphony SP automated DNA processor that can process 1–96 DNA samples per run
- Janus NGS Express Automated Liquid Handling Workstation that automates the fragmentation, labelling, enrichment and amplification of DNA into sequence-ready “libraries” for massively parallel DNA sequencing instruments
- Caliper LabChip GX that automates the electrophoretic analysis of 96 or 384 DNA and RNA samples.
These systems are being used in combination to provide an automated, high-throughput solution to increase sample processing efficiency, ensure standardized sample quality and improve sample tracking. As a result the APF team can now offer human and mouse exome sequencing at a reduced cost to clients. For pricing visit apf.anu.edu.au/genomic-services/exome-sequencing.